2017-8-11 16:0:32025-05-22 15:55:13
_ec
locus
BSU24330
BSU_24330
geneLength
405
408
outlinks
bsu
BSU24330
BSU_24330
The protein
Protein family
asp23 family (according to Swiss-Prot)
asp23 family (with [[protein|YloU]], according to UniProt)
The protein
Paralogous protein(s)
[[protein|YloU]]
[[this]]
Biological materials
Mutant
MGNA-C367 (yqhY::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2365 NBRP B. subtilis, Japan]
GP1468 (Δ''yqhY''::''erm''), available in [SW|Jörg Stülke]'s lab [pubmed|28579978]
GP1765 (Δ''yqhY''::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28579978]
MGNA-C367 (yqhY::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2365 NBRP B. subtilis, Japan]
GP1468 (Δ''yqhY''::''erm''), available in [SW|Jörg Stülke]'s lab [pubmed|28579978]
GP1765 (Δ''yqhY''::''cat''), available in [SW|Jörg Stülke]'s lab [pubmed|28579978]
BKE24330 (Δ[[gene|yqhY]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTCACCTCCGTAA, downstream forward: _UP4_TAAATGGCTTAACACGAAAC
BKK24330 (Δ[[gene|yqhY]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATTCACCTCCGTAA, downstream forward: _UP4_TAAATGGCTTAACACGAAAC
Biological materials
Expression vector
GP1474 (chromosomal ''yqhY''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
pGP1322 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
pGP1325 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
pGP1496 (His-tag, purification from ''E. coli'', in pET28a+), available in [SW|Jörg Stülke]'s lab
pGP1498 (N-terminal His-tag, TEV-site, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
Biological materials
Expression vectors
GP1474 (chromosomal ''yqhY''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
pGP1322 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
pGP1325 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
pGP1496 (His-tag, purification from ''E. coli'', in pET28a+), available in [SW|Jörg Stülke]'s lab
pGP1498 (N-terminal His-tag, TEV-site, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab